Flotillin 1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90519-50, BS90519-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75955(Human) O08917(Mouse) Q9Z1E1(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC FC

All Applications: WB:1:500-1:1,000
IHC:1:50-1:100
FC:1:50-1:100

Background: Lipid rafts are sphingolipid- and cholesterol-rich membrane microdomains that are insoluble in nonionic detergents. Lipid rafts are important for numerous cellular processes, including signal transduction, membrane trafficking and molecular sorting. Flotillins are lipid raft components in neurons and caveloae-associated proteins in A498 kidney cells. Flotillin-1 belongs to the band 7.2/stomatin protein family, whose members are characterized by the presence of a hydrophobic N-terminal region that is predicted to form a single, outside to inside, transmembrane domain. Flotillin-1 and -2 have complementary tissue distributions and their expression levels are independently regulated. At the cellular level, Flotillin-2 is ubiquitously expressed, whereas Flotillin-1 is expressed in A498 kidney cells, muscle cell lines and fibroblasts. Flotillins form a ternary complex with CAP and Cbl, directing the localization of the CAP-Cbl complex to a lipid raft subdomain of the plasma membrane. Association of ER-X with Flotillin localizes ER-X within plasma membrane caveloae and mediates rapid oestrogen activation of the MAP kinase cascade. The expression of the flotillins is also correlated to the progression of Alzhemier pathology.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Flotillin 1 polyclonal antibody detects endogenous levels of Flotillin 1 protein.

Bioworld Molecular Weight: 47 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Flotillin 1 on PC-3M cell lysate using anti-Flotillin 1 antibody at 1/500 dilution., Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.

Alternative Name: FLOT1, FLOT 1, FLOT1, FLOT1, Flotillin-1, Flotillin1, Integral membrane component of caveolae, Reggie 2,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified