GLE1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS75241-50, BS75241-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q53GS7

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of Jurkat cells, using GLE1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 80kDa

Alternative Name: GLE1; GLE1L; LCCS; LCCS1; hGLE1

Immunogen: Recombinant fusion protein of human GLE1(NP_001003722.1).

Conjugate: Unconjugated

Modification: Unmodified