Ki67 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90768-50, BS90768-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P46013(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1,000
ICC:1:200-1:1,000
IHC:1:100-1:500
FC:1:50-1:200

Background: Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization.

Purification and Purity: Peptide affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Ki67 polyclonal antibody detects endogenous levels of Ki67 protein.

Bioworld Molecular Weight: 359 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining Ki67 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Ki67 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Antigen KI-67, Antigen Ki67, MKI67, Antigen identified by monoclonal antibody Ki 67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen KI67, Antigen Ki67, KI67, KIA, Marker of proliferation Ki-67, MIB 1, MIB, MKI67, PPP1R105, Proliferation marker protein Ki-67, Proliferation related Ki 67 antigen, Protein phosphatase 1 regulatory subunit 105, RP11-380J17.2,

Immunogen: Synthetic peptide within Human Ki67 aa 1050-1073.

Conjugate: Unconjugated

Modification: Unmodified