15d-PGJ2 (15-Deoxy-Delta12,14-prostaglandin J2) ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-EL-0087-24T, E-EL-0087-48T, E-EL-0087-96T

Citations, Manuals and MSDS Available upon request.

Abbreviation: 15d-PGJ2

Target Synonyms: 15-d-Δ12; 14-PGJ2;

Research Areas: Signal transduction

Target Species: Universal

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of 15d-PGJ2 concentrations in Serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 1.88 ng/mL

Detection Range: 3.13-200 ng/mL

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 15d-PGJ2. During the reaction, Universal 15d-PGJ2 in the sample or standard competes with a fixed amount of Universal 15d-PGJ2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 15d-PGJ2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 15d-PGJ2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Universal 15d-PGJ2 in samples. No significant cross-reactivity or interference between Universal 15d-PGJ2 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 12 months

Shipping: ice bag