Separase (phospho Ser801) Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA29729-50, RA29729-100

Citations, Manuals and MSDS Available upon request.

Background: extra spindle pole bodies like 1, separase (ESPL1) Homo sapiens Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]) .[supplied by OMIM, Nov 2010],

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage Instructions: -20°C/1 year

Recommended dilutions: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Alternative Names: ESPL1; ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase

Applications: WB; IHC-p; IF/ICC; ELISA

Species Cross-Reactivity: Human; Mouse

GeneID (Human): 9700

Protein MW (KDa): 230

SWISS: Q14674

Source: Rabbit

Research Use Only