SARS-CoV-2 Nucleocapsid Protein IgM ELISA Kit

Size: 96T

Catalogue Number: E-EL-E603-96T, E-EL-E602-96T, E-EL-E601-96T

Citations, Manuals and MSDS Available upon request.

Abbreviation: SARS-CoV-2 IgM

Target Synonyms: COVID 19 IgM; N protein IgM;

Research Areas: SARS-CoV-2, COVID 19

Target Species: Human

Application: ELISA

Usage: This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 IgM in serum or plasma.

Tested Sample Types: Serum, Plasma

Type: Indirect

Assay Time: 1.5h

Detection Type: Colormetric

Test Principle: This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 Nucleocapsid protein IgM in the sample. The micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Nucleocapsid protein (E-ELC-001) antigen, after adding samples to wells, the SARS-CoV-2 Nucleocapsid protein IgM in the samples will combine with the pre-coated SARS-CoV-2 Nucleocapsid protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgM to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Nucleocapsid protein IgM and HRP conjugated anti-human IgM will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Nucleocapsid protein IgM exists in the tested samples or not.

Cross Reactivity: This kit recognizes Human SARS-CoV-2 IgM in samples. No significant cross-reactivity or interference between Human SARS-CoV-2 IgM and analogues was observed

Recovery Rate: 80%-120%

Precision: Intra-CV: CV%＜8%; Inter-CV: CV%＜10%

Storage: 2-8℃, 12 months

Shipping: ice bag