WST-1 is a Tetrazolium dye, widely used in the cell viability assays. WST-1 produces a water-soluble formazan product (WST-1 formazan) that occurs at the cell surface in NADPH dependent manner in viable cells.

Product information

CAS Number: 150849-52-8

Molecular Weight: 651.34

Formula: C19H11IN5NaO8S2

Chemical Name: sodium 5-(2,4-disulfonatophenyl)-2-(4-iodophenyl)-3-(4-nitrophenyl)-3H-1,2λ⁵,3,4-tetrazol-2-ylium

Smiles: [Na+].[O-][N+](=O)C1C=CC(=CC=1)N1N=C(N=[N+]1C1C=CC(I)=CC=1)C1=CC=C(C=C1S([O-])(=O)=O)S([O-])(=O)=O

InChiKey: JUJBNYBVVQSIOU-UHFFFAOYSA-M

InChi: InChI=1S/C19H12IN5O8S2.Na/c20-12-1-3-13(4-2-12)23-21-19(22-24(23)14-5-7-15(8-6-14)25(26)27)17-10-9-16(34(28,29)30)11-18(17)35(31,32)33;/h1-11H,(H-,28,29,30,31,32,33);/q;+1/p-1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 25 mg/mL (38.38 mM; ultrasonic and warming and heat to 80°C). H2O : < 0.1 mg/mL (ultrasonic;warming;adjust pH to 9 with NaOH;heat to 80°C) (insoluble).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

WST-1 is used for a wide range of assays that include cell viability, proliferation, plasma membrane electron transport system, superoxide generation, superoxide dismutase, and xanthine oxidase assay. A water-soluble formazan dye is produced from the reduction of WST-1 tetrazolium, of which optical density at 450 nm is measured to evaluate cell viability.

Products are for research use only. Not for human use.