Human Cortisol ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-EL-0157-24T, E-EL-0157-48T, E-EL-0157-96T

Citations, Manuals and MSDS Available upon request.

Abbreviation: Cortisol

Research Areas: Signal transduction, Metabolism

Target Species: Human

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human Cortisol concentrations in serum, plasma, urine.

Tested Sample Types: Serum, Plasma, Urine

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 2.92 ng/mL

Detection Range: 6.25-400 ng/mL

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Cortisol. During the reaction, Human Cortisol in the sample or standard competes with a fixed amount of Human Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Cortisol. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Cortisol in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human Cortisol in samples. No significant cross-reactivity or interference between Human Cortisol and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 12 months

Shipping: ice bag