EasyStep Human IL6 (Interleukin 6) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK001ES-48, ELK001ES-96, ELK001ES-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: IL-6; MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor

Assay Type: Sandwich

Sensitivity: 0.1 pg/mL

Standard: 100 pg/mL

Detection Range: 1.56-100 pg/mL

Sample Type: serum, plasma

Assay Length: 1.5 h

Research Areas: Cytokine; Tumor immunity; Infection immunity; Cardiovascular biology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL6, and the Human IL6 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL6. After TMB substrate solution is added, only those wells that contain Human IL6 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL6 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P05231

Research Use Only